Figure 5.

Alteration of D2R-DAT protein-protein interaction in AISS rats. A-B,Western blot analysis of the expression levels of D2R dimers (A) and monomers (B) in HEK-293T cells co-expressing D2R/pcDNA3 or D2R/DAT in the presence or absence of AMPH (30 min, 10 μM; n = 3). *Significantly different from D2R/DAT control group (p < 0.05; n = 3). Data were analyzed by t-test. C, Western blot analysis of the expression levels of D2R dimers and monomers in rat striatal slices treated with TAT-fused peptides (30 min, 10 μM) followed by amphetamine exposure (30 min, 10 μM). D-E, Bar graphs summarizing the western blot data. *Significantly different from control group (p < 0.05, n = 3). Data were analyzed by One-way ANOVA, followed by SNK post-hoc test.