Molecular Brain

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Open Access Research

pARIS-htt: an optimised expression platform to study huntingtin reveals functional domains required for vesicular trafficking

Raúl Pardo1,2,3, Maria Molina-Calavita1,2,3, Ghislaine Poizat1,2,3, Guy Keryer1,2,3, Sandrine Humbert1,2,3 and Frédéric Saudou1,2,3*

Author Affiliations

1 Institut Curie, F-91405 Orsay, France

2 Centre National de la Recherche Scientifique, Unité Mixte de Recherche 3306, F-91405 Orsay, France

3 Institut National de la Santé et de la Recherche Médicale, Unité U1005, F-91405 Orsay, France

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Molecular Brain 2010, 3:17 doi:10.1186/1756-6606-3-17

Published: 1 June 2010

Additional files

Additional file 1:

Sequences and maps of pARIS-htt constructs used in the study. The file includes vector maps, DNA sequences, protein translation and additional information for pARIS-mCherry-httQ23/Q100 plasmids in Entry vector. They were generated using Gene Construction Kit (Textco BioSoftware, West Lebanon, USA) and Serial Cloner 2.0 (available at http://serialbasics.free.fr/Serial_Cloner.html webcite.) software.

Format: PDF Size: 899KB Download file

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Open Data

Additional file 2:

Sequence text files of pARIS-htt constructs used in the study. It includes sequences of pARIS-mCherry-httQ23/Q100 plasmids in Entry and pcDNA vectors.

Format: TXT Size: 57KB Download file

Open Data