Figure 6.

CR-proBDNF attenuates amplitude of functional synaptic transmission in cultured hippocampal neurons. Cells were cultured as described in Fig. 5A and treated with the indicated reagents for 3 days. A patch-clamp technique in the whole-cell configuration was used to record mEPSCs from pyramidal-shaped neurons and all data were statistically evaluated. (A) Representative mEPSCs. (B) Amplitude (left) and frequency (right) of mEPSCs. Note that proBDNF significantly decreased the amplitude, but not the frequency, of mEPSCs. Data were collected from 27–31 independent cells (non-parametric test, *P < 0.05, significantly different from Mock).