Molecular genetic analysis of FGFR1 signalling reveals distinct roles of MAPK and PLCγ1 activation for self-renewal of adult neural stem cells

doi:10.1186/1756-6606-2-16

Molecular Brain

Volume 2

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Molecular genetic analysis of FGFR1 signalling reveals distinct roles of MAPK and PLCγ1 activation for self-renewal of adult neural stem cells

Dengke K Ma¹^,2 , Karthikeyan Ponnusamy³ , Mi-Ryoung Song²^,5 , Guo-li Ming¹^,2^,4 and Hongjun Song¹^,2^,4

¹Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

²Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

³Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

⁴Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

⁵Bioimaging Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea

author email corresponding author email

Molecular Brain 2009, 2:16doi:10.1186/1756-6606-2-16


Published:	8 June 2009

Additional files

Additional file 1:

FGF-2 promotes the self-renewal of both rodent and human NSCs. A. Sample images of mouse, rat and human NSCs cultured in the presence of FGF-2 and/or other growth factors. Scale bar: 20 μm. B. Sample images of rat NSCs in various conditions. Scale bar: 20 μm.

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Additional file 2:

Clonal analysis assay. A. A schematic diagram of clonal analysis assay. B. Sample images and quantification of the effect of FGF-2 on clonal expansion. Scale bar: 20 μm.

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Additional file 3:

Defective neuronal differentiation in PLCγ1-depleted cells. Immunostaining of differentiated culture from adult NSCs with PLCγ1 depleted. Shown are Tuj1 (A), DAPI (B), ZsGreen (C) and Merged image (D). Scale bar: 20 μm.

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Additional file 4:

Model. A model of the intracellular signalling mechanisms by which FGF-2 promotes the self-renewal of adult NSCs.

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