In vivo multiplex quantitative analysis of 3 forms of alpha melanocyte stimulating hormone in pituitary of prolyl endopeptidase deficient mice

doi:10.1186/1756-6606-2-14

Molecular Brain

Volume 2

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Warden CH

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Short report

In vivo multiplex quantitative analysis of 3 forms of alpha melanocyte stimulating hormone in pituitary of prolyl endopeptidase deficient mice

Bertrand Perroud¹ , Rudy J Alvarado² , Glenda M Espinal³ , Alex R Morado³ , Brett S Phinney² and Craig H Warden³^,4

¹Genome Center and Bioinformatics Program, University of California, Davis, California, USA

²Genome Center Proteomics Core, University of California, Davis, California, USA

³Rowe program in Genetics, University of California, Davis, California, USA

⁴Section of Neurobiology, Physiology and Behavior, Department of Pediatrics, University of California, Davis, California, USA

author email corresponding author email

Molecular Brain 2009, 2:14doi:10.1186/1756-6606-2-14


Published:	2 June 2009

Additional files

Additional file 1:

PREP in vitro protease activity on α-MSH_1–13produces α-MSH_1–12. Panel A shows the TIC for each α-MSH_1–12MRM transitions (seven left plots) at the beginning of the reaction (T0) and panel B (seven right plots) after one hour incubation (T60). The peak areas after 1 hour of incubation (T60) are about 500 fold of those without incubation (T0), for equivalent injected reaction mix amount.

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Additional file 2:

Detection and quantitation of 3 α-MSH forms using MRM transition methods in pituitary of wild type mouse. The TIC peaks of the (+3) charge state for the 4, 5 and 7 MRM transitions of respectively, deacetylated α-MSH_1–13, acetylated α-MSH_1–13and α-MSH_1–12are shown respectively, in panel A, B and C. Peaks are labeled with the retention time (RT) and the peak area (PA) as calculated with the MS software Xcalibur. The three panels come from a single multiplexed experiment on the same pituitary peptide sample. Panel A shows the 4 MRM transitions of deacetylated α-MSH_1–13. Panel B, the 5 MRM transitions of acetylated, α-MSH_1–13and Panel C the seven transitions for α-MSH_1–12. Quantitation of a compound is calculated by summing the peak areas of all the MRM transitions of a given compound.

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