Additional file 1.

Orexin-A-ir in the mouse hypothalamus, thalamus, and brainstem. Photomicrographs demonstrating regions with moderate to dense populations of orexin-A-ir neurons in the tuberal hypothalamus (arranged rostral to caudal, A to D) and orexin-A-ir fibres in structures of the circadian system including the suprachiasmatic nuclei (E), intergeniculate leaflet (F), dorsal raphe (G), and median raphe (H). 3 V = third ventricle; AHA = anterior hypothalamic area; DMH = dorsomedial hypothalamic area; DR = dorsal raphe nucleus; dLGN = dorsal lateral geniculate nucleus; f = fornix; IGL = intergeniculate leaflet; LHA = lateral hypothalamic area; mlf = medial longitudinal fasciculus; MPA = medial preoptic area; MR = median raphe nucleus; OX = optic tract; PMR = pontine median raphe nucleus; SCN = suprachiasmatic nucleus; TC = tuber cinereum; ts = tectospinal tract; vLGN = ventral lateral geniculate nucleus. Calibration bars = 100 μm (A-D), 50 μm (E, G, H), 25 μm (F).

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Marston et al. Molecular Brain 2008 1:19   doi:10.1186/1756-6606-1-19